Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production
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منابع مشابه
Growth and energetics of Leuconostoc mesenteroides NRRL B-1299 during metabolism of various sugars and their consequences for dextransucrase production.
The metabolic and energetic properties of Leuconostoc mesenteroides have been examined with the goal of better understanding the parameters which affect dextransucrase activity and hence allowing the development of strategies for improved dextransucrase production. Glucose and fructose support equivalent specific growth rates (0.6 h-1) under aerobic conditions, but glucose leads to a better bio...
متن کاملCharacterization of the Different Dextransucrase Activities Excreted in Glucose, Fructose, or Sucrose Medium by Leuconostoc mesenteroides NRRL B-1299.
When grown in glucose or fructose medium in the absence of sucrose, Leuconostoc mesenteroides NRRL B-1299 produces two distinct extracellular dextransucrases named glucose glucosyltransferase (GGT) and fructose glucosyltransferase (FGT). The production level of GGT and FGT is 10 to 20 times lower than that of the extracellular dextransucrase sucrose glucosyltransferase (SGT) produced on sucrose...
متن کاملInduction and transcription studies of the dextransucrase gene in Leuconostoc mesenteroides NRRL B-512F.
Dextransucrase production by Leuconostoc mesenteroides NRRL B-512F in media containing carbon sources other than sucrose is reported for the first time. Dextransucrases were analyzed by gel electrophoresis and by an in situ activity assay. Their polymers and acceptor reaction products were also compared by (13)C nuclear magnetic resonance and high-performance liquid chromatography techniques, r...
متن کاملPurification and Characterization of Dextransucrase from Leuconostoc Mesenteroides Nrrl B-1149
Leuconostoc mesenteroides NRRL B-1149 produces extracellular dextransucrase which in this study was purified using different concentrations of polyethylene glycol (PEG). The dextran produced by this enzyme is unique in that it contains α(16) and α-(13) linkages which have clinical applications. The cell free supernatant with 0.9 U/mg enzyme specific activity was subjected to fractionation by ...
متن کاملAcceptor reactions and mechanism of Leuconostoc mesenteroides B-512FM dextransucrase
14 INTRODUCTION 16 MATERIALS AND METHODS 19 Carbohydrates and reagents 19 Enzyme 19 Enzyme digest conditions 20 Digest conditions to obtain Hanes-Woolf plots 20 Digest conditions to obtain Michaelis plots 20 Determination of the amount of dextran synthesized 21 RESULTS AND DISCUSSION 22 REFERENCES 3 0 iii PAPER II INTERPRETATION OF DEXTRANSUCRASE INHIBITION AT HIGH SUCROSE CONCENTRATIONS 3 9 AB...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1997
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.63.6.2159-2165.1997